Standardization of method for determining glycosylated hemoglobin (Hb A1c) by cation exchange high performance liquid chromatography

Standardization of method for determining glycosylated hemoglobin (Hb A1c) by cation exchange high performance liquid chromatography

Hemoblobin A1c is the most important parameter for the monitoring of metabolic control of patients with diabetes mellitus. The purpose of this study was to adapt the Mono S method to a conventional HPLC system, allowing highly selective HbA1c determination without the acquisition of kits or the use of dedicated systems The results obtained were compared to the Tinaquant® immune turbidimetric me...

Measurment of stable Glycosylated Hemoglobin (Hb A1) and Its unstable type (pre- Hb A1c) with the lon- exchange Chromatography in Diabetic patients

SUMMARY: At present, measurment of glycosylated hemoglobin, especially the stable fraction of hemoglobin (HbA1c) is one of the ways to control diabetes because this measurment can demonstrate total glycemia during last 1.2.3 months. Glycosylated hemoglobin is seen in 3 forms: Ala Alb A1c. Among these hemoglobin Alc is quantitively more than other 2 forms and its difference with other hemoglobi...

Separation of hemoglobin types by cation-exchange high-performance liquid chromatography.

The use of a recently developed cation-exchange HPLC packing material for the separation of hemoglobin types in human blood has been investigated. Adult and newborn hemolysates from normal individuals and from subjects with hemoglobin disorders were analyzed using a weak cation carboxymethyl-bonded phase on 5-micron-particle-size silica. Elution was accomplished using a Bistris (2-[bis(2-hydrox...

Quantitation of hemoglobin A1a+b and hemoglobin A1c by automated "high-performance" liquid chromatography.

The glycosylated hemoglobins A1a+b and A1c have been rapidly and precisely quantitated in 5-micro L samples of human blood hemolysate (approximately 240 micrograms of hemoglobin) by cation-exchange column chromatography. Total chromatographic is 22.0 min. Proportions of Hb A1c range from 3.85 to 6.71% in normal individuals and from 4.23 to 19.90% in diabetic subjects. Within-day variation was 1...

Interference of hemoglobin D on measurements of hemoglobin A1c by the high-performance liquid chromatography HA-8160 in 27 patients.